@Nicola-Silveri I don't know what it could be, if I were you, I would try to rule out the possibilities:
I see that there is a yellow ray being displayed, this indicates that you have a problem with the power source, it needs to be 5V3A (https://www.raspberrypi.org/documentation/configuration/warning-icons.md) Do you have enough space on your SD card? Is your SD card not corrupted? (test with another SD card) I didn’t understand exactly what options you’re changing, I have the impression that you’re creating a new configuration file, and you’re saving to it, but when you exit the game, the emulator is accessing the default configuration file (not the new file), you need to indicate that it is this new file that you want to be used (https://recalbox.gitbook.io/tutorials/configuring-custom-buttons/create-a-custom-configuration-by-emulator), OR, do not save a new configuration file: enter the game, just activate the save option when exiting, exit, open the game again, modify what you want, exit without saving, check.